For some primary cells, transient name : Otsuka Corporation TMSC Data central infrastructure section; handle : OT27-AP; email : [email protected] . Lonza BMNCs are available fresh* or frozen from a large and varied donor pool. In MAbs on 1 July 2019 by Lin, P. C., Chan, K. F., et al.. Srinivasan Kokatam 1 . Data in "No Dox" were determined from all four cell lines not exposed to doxycycline. With the Nucleofector Technology primary cells and stem cells, as well as cell lines, can be consistently transfected at high efficiency. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. Data in "No Dox" were determined from all four cell lines not exposed to doxycycline. This protocol is written for use with the 16-well Nucleocuvette Strips. Actual pmols of RNA and protein may be optimized. Product Overview The Cell Line Optimization 4D-Nucleofector TM X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector TM X Unit. For nucleofection, 4D-nucleofector (LONZA, AAF-1002B, AAF-1002X, AAF-1002Y, and AAF-1002L) transfection was carried out according to the manufacturer's instructions. What benefits are important for your work? This kit has been determined to be optimal for transfecting following cell types: 32D 3T3-L1 pre-ad A20 A375 A7r5 AGS ARPE-19 B16-F10 BA/F3 (DSMZ) C2C12 C6 Capan-1 CHO [suspension] A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A virus infection. Hi Sibel, I also work with B cells and electroporation of big plasmids (approx. The Cell Line Nucleofector TM Kit V is one of our five Nucleofector TM Kits suited for transfecting cell lines in the Nucleofector TM 2b Device. Each dot represents a biological replicate, and Dunn's multiple comparisons test was used to determine P -values. . The machine reportedly includes an updated core unit and intuitive software. Cell therapies have emerged as a promising treatment modality with the ability to augment, repair, or replace human biology, including . . Cell-based immunotherapy uses a cell type from the immune system as therapeutic agent. Moreover, when combined with sleeping beauty-based transposon system, long-term transgene expression could be achieved in all types of cells tested. A suite of cell line development (CLD) services with your priorities in mind for any molecular format CLD requires generating single cell-derived clones that produce high and consistent levels of target therapeutic protein. ZERO BIAS - scores, article reviews, protocol conditions and more . The Cell Line Nucleofector TM Kit V is one of our five Nucleofector TM Kits suited for transfecting cell lines in the Nucleofector TM 2b Device. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. Moreover, Nucleofection is the only electroporation based . FAQ. In Cell Reports on 16 March 2021 by Kulsuptrakul, J., Wang, R., et al.. V4XC-2012 SF Cell Line 4D X Kit L (12 RCT) The SF Cell Line 4D-Nucleofector X Kit L is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 L Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit. difficult-to-transfect cell types, such as suspension cell lines and primary cells Up to 99% transfection efficiency with siRNA duplexes, even in suspension cells Tested for high viability and maintenance of functionality Optimized protocols for hundreds of cell lines and primary cells (see lonza.com) It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates, e.g. Lonza has expanded its Nucleofector line with the 4D-Nucleofector cell transfection platform. The Nucleofector II/2b Transfection Device is the single cuvette based system that has been used in research labs since 2001, and formerly known as the Amaxa device. This kit has been determined to be optimal for . Cell Line Nucleofector Kit V enables expression within hours - from transfection to analysis in a day. The infection only control reflects HIV-1 production in untransfected cells that were infected in parallel. Data presented represent averages from four independent experiments. AsPC-1 and CAPAN-1 pellets were suspended in Lonza transfection reagent (SE Cell Line 4D-Nucleofector X Kit L, Lonza, Germany) when the . https://www.bioz.com/result/cell line 4d nucleofector x kit/product/Lonza Average 93 stars, based on 5 article reviews Price from $9.99 to $1999.99 cell line 4d nucleofector x kit - by Bioz Stars , 2022-08 93 / 100 stars Images Similar Products About News Press Release Team Advisors Partners Contact Bioz Stars Bioz vStars Basel, Switzerland - 2021 marks the 20th anniversary of Lonza's groundbreaking Nucleofector Technology, the industry's leading non-viral cell transfection method, which has been cited more than 10,000 times, including in research by two Nobel Laureates. Sf Cell Line 4d Nucleofector, supplied by Lonza, used in various techniques. This kit has been determined to be optimal for transfecting following cell types: 3T3-L1 ad A-10 BHK-21 BxPC-3 DU145 EL4 FDC-P1 GH3 H9c2 (2-1) HeLa S3 IMR-32 KG-1a L-428 (DSMZ) MDCK Product Overview The Cell Line Nucleofector TM Kit L is one of our five Nucleofector TM Kits suited for transfecting cell lines in the Nucleofector TM 2b Device. Using the Nucleofector II device, we electroporated 14 different cell lines and also primary cells, like mesenchymal stem cells and cord blood CD34+, providing optimized protocols for each of them. View PDF. To determine if an Optimized Protocol is available for your cell type of interest, search our Knowledge Database or contact the Scientific Support Team. I transfected HEK293T with pX458 and pX459 CRISPR constructs using the Lonza Kit V and Q001 program on a nucleofector. . View PDF. View PDF. Category. The MGD Core also offers services to identify induced mutations using High Resolution Melt Analysis (HRMA). Bioassay . Developed in 1998, the Nucleofector Technology was introduced to the research market in 2001 as the first efficient non-viral transfection method for primary cells and hard-to-transfect cell lines. For more than 20 years, Nucleofector Technology has led the market as an effective non-viral cell transfection method, which can be used even for hard-to-transfect cells, such as primary cells and pluripotent stem cells. Introduction. Furthermore, Lonza grants FUJIFILM Cellular Dynamics expanded use of its non-exclusive license to its innovative Nucleofector TM technology, to enable efficient transfection of cells, stem cell and cell lines. An up-to-date list of the continuously extended range of Amaxa Optimized Protocols for the standard Nucleofector Device or the 96-well Shuttle Device can be found on our online cell database at www.lonza.com/cell-database. Once the lead candidate has been identified and a vector generated for use in the selected host cell, the CLD process begins. Description Safety Data Sheet (SDS) Technical Applications COA The Cell Line Nucleofector Kit V is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. Puromycin . Lonza (Figure 1) enable efficient transfection of primary cells and cell lines using a variety of substrates like DNA and siRNA. Human pluripotent stem cell-derived kidney organoids for personalized congenital and idiopathic nephrotic syndrome modeling. This system is designed for maximum fl exibility and enables Nucleofection of cells in several formats combined with advanced performance and convenience. After puromycin selection, those cells went under cell sorting. Transfection performance values are validated in combination For this purpose, the cells are first removed from the body, then activated or modified, expanded and finally re-infused into the patient. The stable cell line . T cells were electroporated (Amaxa Nucleofector 2b, Amaxa Human T Cell Nucleofector Kit, Lonza, Germany) with 3 g sgRNA-BCL11B plasmids for 1 107 cells . Nucleofector - Amaxa 2b Transfection device with over 160 protocols optimized by Lonza's R&D Team for cell lines and primary cells and more than 680 entries in the online Cell Transfection Database , Nucleofector Technology is the transfection method of choice for difficult-to-transfect cell types. HeLa, IMR-90, NIH/3T3, NK-92, or Sf9, in the Nucleofector TM I/II/2b Device Size These are some of the strategies currently being investigated: Adoptive T cell therapy focuses on . Lonza has launched the next-generation of its popular Nucleofector Platform. V4SP-5096, V4XP-5032, or V4XP-5012 depending on the . Immunoreactivity of humanized single-chain variable fragment against its functional epitope on domain 1 of CD147. You've come to the right place! In case no data are shown for the selected Nucleofector Platform, please take a look at our optimization strategy to get further guidance on how to easily . Set up the RNP formation reaction as follows below. The Amaxa nucleofector uses a novel non-viral transfection technology to introduce nucleic acids (both DNA and RNA) into stem cells, primary cells, and cell lines that are difficult to transfect. The innovative Nucleofector Technology enables the transfer of nucleic acids and proteins directly into the cells' nuclei by . Cells were analyzed 24 hours post Nucleofection using light (A) and fluorescence microscopy (B). Isolating mononuclear cells eliminates erythrocytes and graulocytes, producing a more stable cell product. 6 Based on numerous user feedback, Lonza engineers and scientists have developed the new innovative 4D-Nucleofector System. Lonza generated sales of CHF 3 billion with a CORE EBITDA of CHF 987 million in H1 2022. The basic Nucleofector II Device is a single cuvette based system that has been used in research labs since 2001. It is based on two components: the Nucleofector Device that delivers unique electrical parameters, and Nucleofector Kits that contain cell-type-specific Nucleofector Solutions. Each transfection is performed with the Lonza SE cell line 4-D nucleofector solution kit by electroporating one million HCT116 cells in an electro-cuvette. Cells were analyzed 5 hours post Nucleofection using light (A) and fluorescence They are guaranteed to contain either . Lonza is a verified supplier Lonza manufactures this product About 96 Buy from Supplier Structured Review Lonza cell line nucleofector kit v Cell Line Nucleofector Kit V, supplied by Lonza, used in various techniques. Both DNA and RNA are efficiently transfected into both the cytoplasm and . The Lonza shares are listed on the SIX Swiss Exchange and Swiss Market Index (SMI). Furthermore, Lonza grants FUJIFILM Cellular Dynamics expanded use of its non-exclusive license to its innovative Nucleofector technology, to enable efficient transfection of cells, stem cell and cell lines. Now, with an updated core unit and even more intuitive software, the next-generation 4D . Informatics for QC Microbiology . The Lonza 4D-Nucleofector platform has multiple electroporation volume and vessel options. We also maintain a secondary listing on the SGX Singapore Exchange. Lonza's 4D-Nucleofector System (Fig. Cryopreserved Lonza Bone Marrow Mononuclear Cells are isolated from whole bone marrow via density gradient separation. It employs a non-viral method based on an optimized combination of electrical parameters and cell-type specific solutions. with 0.4 g pmaxGFP Vector using the P5 Primary Cell Nucleofector Kits (Lonza, Cat. Prepare the working Nucleofector solution by mixing SF cell line 96-well Nucleofector solution with the entire contents of the enclosed supplement tube. Muenchensteinerstrasse 38 CH-4002 Basel Switzerland Business Telephone +41 61 316 81 11 Lonza Inc. - Rockland 191 Thomaston Street Rockland, ME 04841-2994 USA Business Telephone: 1-207-594-3400 Lonza Inc. - Walkersville 8830 Biggs Ford Road Walkersville, MD 21793-0127 USA Business Telephone 1-301-898-7025 Bioz Stars score: 96/100, based on 1 PubMed citations. Nucleofector Supplement is added to the Nucleofector Solution it is stable for three months at 4C. Nucleofector Supplement is added to the Nucleofector Solution it is stable for three months at 4C. Lonza provides cell type-specific 96-well Amaxa Optimized Protocols for many different cell lines and primary cells. Nucleofector Technology primary cells and stem cells, as well as cell lines, can be consistently transfected at high effi ciency. In addition, our Knowledge Database contains transfection data for close to 1500 cell types. Protocol: Electroporation Seed the cells so that they will be around 70-90% confluent on the day of transfection. 10 kbp) by using Amaxa Nucleofector I (Lonza) along with the Cell Line Nucleofector Kit V . SH-SY5Y cells were transfected with the Cell Line Nucleofector Kit V, Program A-023 and 2 g of a plasmid encoding the enhanced green fluorescent protein eGFP. Cell Line Nucleofector Kit L VCA-1005 Cell Line Nucleofector Kit L Product Code 191669 Product Pack Code PC Stocked Overseas Stocked Item Sign In To Purchase Description Safety Data Sheet (SDS) COA For efficient transfection of cell lines, e.g. View PDF. In The Journal of Biological Chemistry on 18 August 2022 by Economos, N. G., Thapar, U., et al.. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Cell Line Nucleofector Kit R | Lonza Pharma & Biotech Home Products Transfection Cell Line Nucleofector Kit R Save to list Cell Line Nucleofector Kit R 1 of 3 Nucleofector I/II/2b Kit (25 reactions) Catalog #: VCA-1001 For efficient transfection of cell lines, e.g. Transfection of any cell With over 160 protocols optimized by Lonza's R&D team for cell lines and primary cells and more than 680 entries in the online cell database, Nucleofector Technology is clearly the transfection method-of-choice for any difficult-to-transfect cell type. Store excess complete Nucleofector solution at 4 C for up to 3 months for future use. The 4D-Nucleofector X Unit supports transfection in two different formats. This method uses a guide RNA to protein ratio of 10:1. ZERO BIAS - scores, article reviews, protocol conditions and more This kit has been determined to be optimal for transfecting following cell types: B16-F0, BJ, COS-7, HeLa, HT-29, Hut-78, IMR-90, KG-1, L6, LNCaP, MDBK, NIH/3T3, NK-92, PANC . Manufacturer: Lonza Walkersville VCA1001 The Cell Line Nucleofector Kit R is one of five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. Harris Computer: clinical data partnership Lonza: 4D-Nucleofector cell transfection platform Tools4Patient: Placebell placebo-response technology Clinical Ink: eCOA . Here we present a method for the introduction of Cas12a RNP's into HEK293 FT cells using the Lonza 4D-Nucleofector Electroporation System. Our Early Development Services experts will work with you to customize your assessment strategy based on your therapeutic goal and timeline. Lonza Group may not be able to comply with your request to delete your PI under the circumstances as described below, under which Lonza Group shall stop all data processing except data storage and necessary data protection actions: Within the archive term required by relevant laws or administrative regulations. . HepG2 cells were transfected with the Cell Line Nucleofector Kit V, Program T-028 and a plasmid encoding the mouse MHC class I heavy chain molecule H-2K k. Cells were stained 24 hours post Nucleofection with a PE-coupled antibody Our Leadership Board of Directors Executive Committee Other Committees Endotoxin Detection. Cells were reprogrammed by episomal overexpression of SOX2, OCT3/4, KLF4, LIN28, L-MYC, and TP53-shRNA (2). In Scientific Reports on 25 April 2022 by Intasai, N., Rangnoi, K., et al.. Register a free CiteAb account today to view them. The electrical parameters of the Nucleofector are different from any other . Bioz Stars score: 95/100, based on 1 PubMed citations. TF-1 cells were transfected with the Cell Line Nucleofector Kit T, Program T-001 and 2 g of pmaxGFP Vector. Immunogenicity Platform. Citations. Browse: Cell and Transfection, . Attenuated glutamine synthetase as a selection marker in CHO cells to efficiently isolate highly productive stable cells for the production of antibodies and other biologics. No. Brand: Lonza VCA-1003 Additional Details : Weight : 0.50400kg 3D Cell Culture . The Nucleofector technology is a highly efficient non-viral method for transfection. Nucleofector Solution 2.2 Start 4D-Nucleofector System and create or upload experimental parameter file (for details see device manual) 2.3 Select/Check for the appropriate Nucleofector Program (see table 3) 2.4 Prepare cell culture plates by filling appropriate number of wells with desired volume of recommended culture media (see table 4) If no transfection data are available, we have listed the Nucleofector Kit recommended for optimization of this cell type. Cell therapies have emerged as a promising treatment modality with the ability to augment, repair, or replace human biology, including . In Development (Cambridge, England) on 1 May 2022 by Jansen, J., van den Berge, B. T., et al.. Patient-specific, re-engineered cardiomyocyte model confirms the circumstance-dependent arrhythmia risk associated . Lonza Optimized Protocol Optimization Guideline Filter: The table below shows data for the cell type and Nucleofector Platform selected. DNA vectors or siRNA oligonucleotides, in low throughput format. Each dot represents a biological replicate, and Dunn's . An ELISA-based platform for rapid identification of structure-dependent nucleic acid-protein interactions detects novel DNA triplex interactors. Data was normalized to cell numbers . Cell Services. Cell Lines and Primary Cancer Cells. Classical Media and Reagents. www.lonza.com/celldatabase Transfection performance data is based upon both data from Lonza R&D and on customer data. 3T3-L1 ad, DU145, EL4, IMR-32 or MDCK, in the Nucleofector I/II/2b Device Allow the mixed reagents to equilibrate at room temperature prior to use. This kit has been determined to be optimal for transfecting following cell . Solutions. Nucleofector Supplement is added to the Nucleofector Solution it is stable for three months at 4C. Transfection of any cell With over 160 protocols optimized by Lonza's R&D team for cell lines and primary cells and more than 690 entries in the online cell database, Lonza. TALEN and Crispr-Cas9 DNA nucleases are a cutting edge technology for performing reverse genetic studies in multiple model systems, including, but not limited to Zebrafish, Drosophila, C. elegans, Mouse, and mammalian tissue culture. Developed in 1998, the Nucleofector Technology was introduced to It allows efficient transfection of hard-to-transfect cell lines and primary cells for transfection in low-throughput format with: The Nucleofector II/2b Device can also . The table below shows data for the cell type and Nucleofector Platform selected. SF Cell Line 4D-Nucleofector X Kit (Cat # V4XC-2032) Sterile 1X PBS without Ca 2+ and Mg 2 . 3.5 x 10 6 cells were electroporated with 7 g expression plasmid mixture using a 4D Nucleofector (Lonza, program EO117) and the P3 Primary Cell 4D-Nucleofector X-Kit (#V4XP-3032, Lonza), according to the manufacturer's instructions. Shop Lonza Walkersville SF Cell Line 4D-Nucleofector X Kit L, 12 reactions, 100uL Nucleocuvette Vessels at Fishersci.com Electrophoreses and Analysis . As part of Nucleofector Technology, Lonza provides cell-type speci!c Optimized Protocols for many di"erent cell lines and primary cells. Culture Media. Our Epibase platform of in silico immunosafety profiling and in vitro cell based assay services evaluate the immunogenicity risk potential of your biotherapeutic candidates. . SE Cell Line 96-well Nucleofector Solution 0.675 ml SF Cell Line 96-well Nucleofector Solution 0.675 ml SG Cell Line 96-well Nucleofector Solution 0.675 ml Supplement 30.150 ml pmaxGFP Vector (1.0 g/l in 10 mM Tris pH 8.0) 50 g Nucleocuvette Plate(s) 1 Storage and Stability Bioprocess Containers. Karolinska Institutet. Need technical data? Cells are plated and cultured for 48 h post transfection (positive cells are depicted as gray with a green ring around the nuclear lamina). Basel, Switzerland, 07 April 2021 - 2021 marks the 20th anniversary of Lonza's groundbreaking Nucleofector Technology, the industry's leading non-viral cell transfection method, which has been cited more than 10,000 times, including in research by two Nobel Laureates. Amaxa Nucleofector Technology - Maintenance of Functionality Nucleofector Technology - High Efficiency and Functionality The Amaxa Nucleofector Technology has become the method of choice when transfection of primary cells or difficult-to-transfect cell lines is required.